Fast Flow强阳离子交换凝胶色谱结合的方法对复合果胶酶制剂Pc-3中的果胶酯酶.(PE)和聚半乳糖醛酸酶(PG)进行分离。超滤后,分别在0 25ml/min～1.00ml/min洗脱流速下进行0～1mol/L NaCl的梯度洗脱,得到两个部分。随着洗脱流速的降低,第一部分的PE和PG酶活比逐渐上升,由1.49增加到了3.69,在流速0.25ml/min的洗...
PROBLEM TO BE SOLVED: To provide a product forming method capable of shearing a product over its whole circumference by a progressive die.;SOLUTION: A scrap holder 15 is lowered together with a punch 13, and a circumferential edge part 23 of a preliminarily formed part 22 located around the...