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(25 µg/mL) at 37 °C and 5% CO2. Two hours later, PE-Mφ were washed three times to remove the non-phagocytosed antigen. Splenocytes from WT mice infected withT. cruzifor 21 days were added at a ratio of 1:10 (Mφ: splenocytes; 100 µL of splenocytes adjusted to...