To quantitatively measure Ubc9 diffusion in spines, FRAP data were individually fitted with a single-phase exponential function to obtain values for the mobile fraction and the rate of fluorescence recovery (half-time of recovery; see the Methods section for details). The mobile fraction is the ...
Two mice in each group were randomly selected and analysed in this study. The investigator was blinded to the group allocation during the experiment. Paraffin-embedded tumours were sectioned into 4 μm sections by Leica RM2255 microtome (Leica, Wetzlar, German). The sections were rehydrated in ...