Free-floating sections were incubated for 48 h at 4°C in a rabbit anti-NPY antiserum (Amersham AB, Buckinghamshire, England) diluted at 1 : 2000; then rinsed in PBS and processed by the avidine–biotine peroxidase complex method using an ABC-peroxidase kit (Vector Lab). The specificity ...
PTX (a–c), the voltage-dependent calcium channel (VDCC) blocker nifedipine (Nife., d–f) or CPA, a potent inhibitor of the Ca2 þ -ATPase pumps (d–f), the PLC inhibitor U73122 (g–i) and the PKC inhibitor chelerythrine (Cheler., g–i) as described in the Methods section. Ne...
c To find out the role of mGlu8 in the expression of morphine -induced CPP, the animals received highest dose of S-3,4-DCPG (3 μg/0.5 μL saline) or Saline 5 min prior to CPP test Full size image Pre-conditioning phase On day 1, each rat was separately placed in the apparatus...
After an incubation for 25 min at room temperature under shaking, beads were washed three times in t-PBS and then resuspended in SDS-PAGE loading buffer. Samples were boiled at 95 °C for 5 min to elute proteins from beads and subjected to Western blot analysis (see section Immunoblotting)...
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