A simplified mGlu receptor dimer activation model and its domains: VFD - Venus Flytrap Domain; CRD - cysteine-rich domain; 7TMD - transmembrane heptahelical domain, shown as a cylinder with three highlighted transmembrane helices (TM 4–6) within the lipid bilayer and the intracellular C-terminus...
Neurons were treated or not with 10 mM bicuculline, 2 mM TTX or 50 mM DHPG in Earle's buffer (25 mM HEPES-Tris pH 7.4, 140 mM NaCl, 5 mM KCl, 1.8 mM CaCl2, 0.8 mM MgCl2, 0.9 g l À 1 glucose) for 10–20 min in Earle's buffer. A 10–20 min preincubation at 37 °C ...
c To find out the role of mGlu8 in the expression of morphine -induced CPP, the animals received highest dose of S-3,4-DCPG (3 μg/0.5 μL saline) or Saline 5 min prior to CPP test Full size image Pre-conditioning phase On day 1, each rat was separately placed in the apparatus...
Eluted fractions were analyzed through immunoblot assay (see Western blot section). The immunoreactivity of mGlu2 receptors was monitored in the total lysate (L), in control (C), in MDL11,939-treated and clozapine-treated biotinylated synaptosomes, and in the streptavidin pull-down of the non-...
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