cDNAs were synthesized using the adaptor-ligated mRNAs as template using a modified small RNA reverse transcriptase (RT) primer from Illumina (5′-CAAGCAGAAGACGGCATACGANNNNNNNNN-3′) and Superscript II Reverse Transcriptase (Life Technologies, Carlsbad, CA). The RNA was mixed with 25 μM ...
pneumoniae MGH 78578 using specifically designed primers. A specific amplicon of 657 bp was obtained (Figure 6(a)). Cloning of this amplicon into pGEM®-7zf (+) was subsequently achieved, as confirmed from blue-white screening (data not shown), colony PCR (Figure 6(b)) and sequencing ...