In this study a possible association between (Ca(2+)-Mg2+)-ATPase activity and the neural cell adhesion molecule, NCAM, was investigated. The effects of various detergents on ATPase activity were evaluated, and it was found that solubilization of rat brain microsomes with 3-[(3-cholamidopropyl...
神经节苷脂(Gangliosides)是红细胞膜Ca2+-Mg2+ATPase的一种激活剂,这种激活作用也是依赖于Ca2+存在.在200μmol/LCa2+存在的反应体系中,100μg/mLGangliosides对Ca2+-Mg2+ATPase的激活作用最大,为基本酶活性的150%以上.实验还发现CaM拮抗剂三氟拉嗪(TFP),粉防已碱(Tet)等也同样抑制Gangliosides的这种激活作用....
Highly coupled ATP synthesis by F1-ATPase single molecules In the ubiquitous F0F1 complex, this energetically uphill reaction is driven by F0, the partner motor of F1, which forces the backward (clockwise) ... Yannick,Rondelez,Guillaume,... - 《Nature》 被引量: 547发表: 2005年 Phosphatidylin...
因为Mg2+是DNA聚合酶(Taq聚合酶)的必需激活剂!Mg2+浓度越高,聚合酶活性越强,过强就会出现非特异性扩增!Mg2+浓度越低,聚合酶活性越低,扩增效率也就越低,产物就越少!=== 所以PCR扩增实验,需要根据实际情况,摸索适宜的Mg2+浓度!
催化作用,用于激活酶的活性
The effect of Mg2+ on ATP-activated current in rat nodose ganglion neurons was investigated with the use of the whole cell patch-clamp technique. Mg2+ decreased the amplitude of ATP-activated current in a concentration-dependent manner over the concentration range of 0.25-8 mM, with a 50% in...
1、血清镁增高 (1)肾脏疾病凡影响肾小球滤过率者均可使血清镁滞留而增高。如慢性肾炎少尿期、尿毒症、急性或慢性肾功能衰竭等。 (2)内分泌疾病如甲状腺功能减退症(黏液性水肿)、甲状旁腺功能减退症、阿狄森病、未治疗的糖尿病昏迷(治疗后迅速下降)。(3)治疗措施不当
The streaming which had been stopped in a medium containing no ATP could be reactivated by the Mg · ATP-containing medium and continued for more than one hour. The rate of the reactivated streaming for the first 30 minutes was almost the same as that of the normal streaming. The ...
甘草黄酮对大强度耐力运动大鼠肾脏组织Ca2+、Mg2+-ATPase及Bax、Bcl-2蛋白表达的影响