MG132,3-Methyladenine(3-MA),NH<Sub>4</Sub>Cl均购买与Sigma公司,其中临用时将26.745g的NH<Sub>4</Sub>Cl固体溶于100ml过滤无菌水中,充分溶解配成母液为5M>4</Sub>Cl溶液,用时1:200稀释。 实施例1 MG132对FMDV结构蛋白VP3表达的影响 1.1实验步骤 (1)HEK-293T细胞铺于12孔板,12h后共转染pCDNA3.1-...
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AO染色和Westernblot法检测自噬的改变.结果:MG132可诱导A2870卵巢癌细胞发生增殖抑制及凋亡,同时,酸性自噬泡形成及LC3-I向LC3-II的转化增强.而3-MA联合MG132明显降低A2870细胞的生存率,但不改变MG132诱导的A2870细胞的自噬水平.结论:3-MA对MG132抗A2870卵巢癌细胞的增强作用与自噬无关.MG132诱导A2870细胞发生的...
3 PowerPoint slide for Fig. 4 PowerPoint slide for Fig. 5 PowerPoint slide for Fig. 6 Rights and permissions Reprints and permissions About this article Cite this article Yan, H., Ma, Yl., Gui, Yz. et al. MG132, a proteasome inhibitor, enhances LDL uptake in HepG2 cells in vitro ...
蛋白酶体抑制剂MG132对外周血内皮祖细胞数量与功能的影响
蛋白酶体抑制剂MG132对早期糖尿病肾病大鼠肾脏MKP-1表达的影响
Results: Treatment of HepG2 cells with the specific proteasome inhibitor MG132 (0.03–3 μmol/L) dose-dependently increased LDLR mRNA and protein levels, as well as LDL uptake. Short-term treatment with MG132 (0.3 μmol/L, up to 8 h) significantly increased both LDLR mRNA and protein ...
Figure 3. MG132 pre-treatment decreases on the mRNA level of fibrosis-associated factors in NRK-49F cells simulated by TGF-β1 in a concentration-dependent manner. The mRNA levels were normalized to the control group. The cells were treated with the proteasome inhibitor, MG132, at specific ...
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蛋白酶体抑制剂MG132对Raji细胞凋亡、细胞周期及UBE1基因表达的影响