Signals were detected using a GeneGnome XRQ analysis system (SYNGENE, Hyderabad, India) and analyzed using Image Gauge V4.0. 2.5. cGMP ELISA The samples were homogenized in 5% TCA in water and centrifuged at 3000× g for 5 min. The levels of acetylated cGMP in the supernatant were ...
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