MFC-MRD检测阳性有助于识别BCP ALL复发高风险人群,而很多MFC-MRD阴性的患者也会复发。因此,亟需更灵敏的检测技术以更准确的识别具有高复发风险的人群,尤其针对MFC-MRD阴性的患者。 研究方法 研究共纳入93例BCP ALL患者,取所有患者初诊时和巩固化疗2个周期后的配对骨髓样本,初诊时的骨髓样本进行NGS MRD检测(SeqMRD...
Minimal residual disease (MRD) refers to the residual leukemia cells that persist after chemotherapy. Patients who test positive for MRD have a higher likelihood of experiencing a recurrence, regardless of the specific chemotherapy approach used. Multi‐parameter flow cytometry (MFC), polym...
为了评估MRD的结果是否可信,我们分别计算了在MRD阈值为1×10−5时,每次检测的MRD阴性病例和阳性病例的比例,以及血清学状态。结果显示,两种技术获得的MRD检测结果与血清学反应完全一致(表1)。 表1 MRD阳性病例的T细胞负荷与这两种检测结果相符 在MRD阈值为1×10−5时分别对58例和56例NGS/MFC MRD阳性病例进行比...
MFC可以识别每个患者独特的LAIP,并将其作为该患者的MRD在后续样本检测中进行追踪,可广泛应用于90%的AML患者,且采用MFC检测MRD的灵敏度较高。 国内外多项研究肯定了MRD在AMLmCR患者中的预后意义[7,8,9,10]。FREEMAN等[7]使用MFC评估2450例成年AML患者MRD的研究指出,首次诱导化疗后MRD阳性患者与化疗后部分缓解患者...
目的:探索应用多参数流式细胞术(MFC)监测儿童急性髓系白血病(AML)诱导化疗后骨髓微小残留病(MRD)对预测预后的价值.方法:回顾性分析2015年8月-2022年12月武汉儿童医院... 卢文婕,熊昊,杨李,... - 《中国实验血液学杂志》 被引量: 0发表: 2024年
Background: MFC is one of the reference methods for MRD assessment in AML. However, when compared to molecular approaches, MFC lags behind in specificity and sensitivity. Of striking note is the fact that common markers used in MFC were established decades ago at times of limited instrument and...
目的采用多参数流式细胞术(MFC)检测细胞膜表面标记和实时荧光定量(qPCR)术检测WT-1基因表达,评估急性白血病治疗后微小残留病灶(MRD)水平及对预后影响.方法 收集2018-2020年诊断的急性白血病患者52例,女性32例,男性20例,平均年龄53岁(年龄分布14~76岁),其中ANLL 43例,ALL 9例,分为初诊组,化疗组,缓解组.MFC监测...
All cases were referred for MRD assessment by MFC at day+100 after HDT/ASCT; baseline FISH analysis were available in 110 of the 241 patients.We first investigated which of the most relevant disease characteristics had prognostic influence in patients in CR at day+100 a...
更多“MFC技术检测MRD的优点是()”相关的问题 第1题 AML患者的MRD监测,临床医生建议RT-PCR,MFC和NGS三种方法同时采用。这是因为() A.三种方法采用的标志物不同,互为补充 B.三种方法同时采用,可以提高MRD检测的准确性 C.三种方法同时采用,可以更早预测复发 D.三种方法同时采用,可增加患者经济负担 E.三种方法...
The best-fit MRD cut-off was chosen as 1 × 105, resulting in an overall 9.6% (n overall = 12 (NGS n = 2, MFC n = 10)) nonassessable cases. The overall concordance rate between NGS and MFC was 68.0% (n = 85); discordant results were found in 22.4% (11.2% (n = 14) of ...