1) MexXY-OprM MexXY-OprM外排泵 1. AIM:To study the impact of glutathione synthetase(gshb)gene mutant of Pseudomonas aeruginosa on the expression ofMexXY-OprMefflux pump. 目的:研究铜绿假单胞菌谷胱甘肽合成酶基因(gshb)突变体对MexXY-OprM外排泵表达的影响。
MexXY-OprM多重耐药目的探讨MexAB-OprM和MexXY-OprM主动外排系统与铜绿假单胞菌多重耐药的关系。方法挑选临床分离的铜绿假单胞菌敏感株和多重耐药株各30株,以及标准质控株,用RT-PCR扩增其mexB、mexY和核糖体参照基因rpsL片段,根据mexB、mexY与rpsL扩增产物的电泳扫描比值,分析mexB与mexY mRNA的表达水平。结果标准...
排系统MexAB-OprM、MexXY-OprM在多重耐药铜绿假单胞菌中过度表达,是此菌多重耐药机制之一; 外排泵Mex_M~OprM、MexXY-OprM高表达分别与调控基因m sxR、mexZ发生变异有关。同时存在其他 调控机制。 【关键词】多重耐药;铜绿假单胞菌;主动外排系统(外排泵) ...
铜绿假单胞菌MexAB—OprM、MexXY—OprM主动外排系统的耐药作用
In this study, we investigated the implication in the phenomenon of MexXY-OprM, an active efflux system known to export aminoglycosides in P. aeruginosa. Immunoblotting experiments demonstrated that the transporter MexY, but not the outer membrane pore OprM, was overproduced during the post-drug ...
Berberine inhibits the MexXY-OprM efflux pump to reverse imipenem resistance in a clinical carbapenem-resistantPseudomonas aeruginosaisolate in a planktonic state... F Su,J Wang - 《Experimental & Therapeutic Medicine》 被引量: 0发表: 2018年 Berberine inhibits the MexXYOprM efflux pump to reverse ...
We used different classes of EPIs for MexAB- or MexXY-OprM to measure the accumulation of various fluorescent dyes, including SYBR Safe, AtlasSight, and GelGreen. Methods Escherichia coli MG1655ΔacrBΔtolC strain harboring plasmids carrying the mexAB-oprM (pABM) or mexXY-oprM (pXYM) genes ...
(P <0.05 ).Conclusion In multidrug resistant pseudomonas aeruginosa,the over-expression of MexXY-OprMplays an important role.%目的:研究 MexXY-OprM主动外排系统在铜绿假单胞菌(PA)多重耐药机制中的作用.方法挑选临床分离的PA 多重耐药菌株26株,敏感菌株15株及标准质控菌株(ATCC27853),用 RT-PCR 方法扩增...
MexAB-OprMMexXY-OprM多重耐药目的 探讨MexAB-OprM和Mex XY-OprM主动外排系统与铜绿假单胞菌多重耐药的关系.方法 挑选临床分离的铜绿假单胞菌敏感株和多重耐药株各30株,以及标准质控株,用RT-PCR扩增其mexB,mexY和核糖体参照基因rpsL片段,根据mexB,mexY与rpsL扩增产物的电泳扫描比值,分析mexB与mexY mRNA的表达...
Simultaneous overexpression of the MexAB-OprM and MexXY efflux systems was demonstrated by real-time reverse transcription-PCR and immunoblotting experiments for 12 multiresistant clinical isolates of Pseudomonas aeruginosa. DNA sequencing analysis showed that nine of these strains (named agrZ mutants) har...