Here, we generated transgenic mice lacking RNA methyltransferase-like 3 (Mettl3) specifically in Pdx1+ pancreatic progenitor cells and found the mice with the mutation developed hyperglycemia and hypoinsulinemia at age 2 weeks, along with an atrophic pancreas, reduced islet mass, and abnor...
For each mutation position, the coverage of a unique tag (k) and mutations (m) was determined by the CIMS. pl program [67]. The positions within k ≥ 15 and m/k ≤ 50% were kept and only mutation positions within the RRACH motif were identified as m6A for downstream analysis to ...
Given that mice with a conditional mutation for either Mettl3 or Mettl14 show normal meiosis and sper- miogenesis, these results indicate that these two enzymes could have different or partially overlapping functions in late spermatogenesis. This observation also suggests the presence of additional ...
Although both HNSC and CESC were HPV-related squamous cell carcinoma, the mutation spectrums were not the same. Initially, we summarized the distribution of different types of mutations in HNSC and CESC (Figure S5A). Other types of mutations (65.88% samples) and missense substitution (60.14% ...
Detection of epidermal growth factor receptor gene mutation in different sample types of 238 patients with non-small cell lung cancer [J]. Journal of Modern Laboratory Medicine, 2018, 33(4): 31-33. [2] TAN K S. Misclassification of the actual causes of death and its impact on analysis: ...
d mRNA level of Firefly luciferase in HEK293T cells transfected with empty pMIR (Vector) or plasmids bearing wild-type (Wt) Tirap 3′-UTR (left) or CD40 3′-UTR (middle) either, or their m6A site mutation 3′UTR (Mut). The results were normalized by Renilla luciferase and presented ...
METTL3 catalyzes the formation of N6-methyl-adenosine (m6A) which has important roles in regulating various biological processes. However, the in vivo function of Mettl3 remains largely unknown in mammals. Here we generated germ cell-specific Mettl3 knoc
2G). Then the plasmids with the A-to-G mutation at the 446, 451, 10,631 and 10,709 base sites of WT were constructed (Fig. 2H). Subsequently, MeRIP-qPCR indicated that m6A levels of GBAP1 were significantly reduced both in Hep3B and MHCC97H cells with ectopic expression of the GBA...
Luciferase reporter assay showed that the transcriptional level of wild-type ZMYM1, but not the mutation, significantly decreased in the absence of METTL3 (Fig. 6d). Reciprocally, METTL3 overexpression augmented the expression of wild-type ZMYM1-fused reporter, but failed to influence the ...
Oridonin inhibits DNMT3A R882 mutation-driven clonal hematopoiesis and leukemia by inducing apoptosis and necroptosis Article Open access 18 October 2021 Data availability The datasets related to STM2457 used in this study can be accessed from the European Nucleotide Archive under accession PRJEB4166...