Cellular senescence is characterized by a decrease in protein synthesis, although the underlying processes are mostly unclear. Chemical modifications to transfer RNAs (tRNAs) frequently influence tRNA activity,
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Questions answered in this articleBetaPowered by GenAIThis is generative AI content and the quality may vary. Learn more. What mechanisms are involved in the regulation of inflammation and apoptosis in trophoblast cells by HOXD-AS1? How does the METTL3/HOXD-AS1/miR-135a/β-TRCP axis influence...
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In consideration of the questions mentioned above, in this study, we aimed to investigate the role and underlying mechanism of miR-338-5p in lung cancer. Our research showed the significant downregulation of miR-338-5p in lung cancer tissue and cells and further revealed that miR-338-5p inhibi...
(UCN)) and significance calculated using Tukey’s multiple comparisons test.gIn vitro transcribed mt-tRNAThrand mt-tRNASer(UCN)were incubated with purified His14-MBP-METTL8 and [3H]-SAM. Tritium incorporated into the RNA was measured by scintillation counting. Bar plots show mean counts per ...
Statistical analysis was performed using one-way ANOVA (F = 35.18, p < 0.0001 for mt-tRNAThr; F = 36.04, p < 0.0001 for mt-tRNASer(UCN)) and significance calculated using Tukey's multiple comparisons test. g In vitro transcribed mt-tRNAThr and mt- tRNASer(UCN) were incubated with ...
Whether the reduced enzyme amount led to less modified ribosomes in the aged tissues, and if these are natural processes in place to respond to senescence-related stresses, are important questions that will require further investigations. Recently, familiar recessive mutations in METTL5 gene were ...
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