Methylmalonic acid quantification in low serum volumes by UPLC-MS/ MS. J Chromatogr B Analyt Technol Biomed Life Sci 2011;879:1502-6.Pedersen TL, Keyes WR, Shahab-Ferdows S, Allen LH, Newman JW (2011) Methylmalonic acid quantification in low serum vol- umes by UPLC-MS/MS. J Chromatogr ...
METHODS: Deuterated internal standard d(3)-MMA was added to serum or plasma before SPE on strong anion-exchange (SAX) columns. After elution with HCl-butanol (10:90 by volume) and addition of 1 g/L formic acid, the samples were simultaneously derivatized and evaporated by heating to 70 ...
Introduction: Methylmalonic acid (MMA), a metabolite and by-product of propionate metabolism, promotes breast cancer (BC) progression in mice via the transforming growth factor-beta (TGF尾) signaling pathway (Gomes et al, Nature 2020). It is currently unknown if this effect also exists in ...
Measurement of methylmalonic acid (MMA) plays an important role in the diagnosis of vitamin B12 deficiency. Vitamin B12 is an essential cofactor for the enzymatic carbon rearrangement of methylmalonyl-CoA (MMA-CoA) to succinyl-CoA (SA-CoA), and the lack
Methylmalonic Acid Performance Goals To be able to detect vitamin B 12 deficiency, methods should be able to accurately measure concentrations of methylmalonic acid of approximately 1 µmol/L in urine and 0.1 µmol/L in serum. The GC method has good precision (coefficient of variation [CV]...
Kushnir, M.M., Nelson, G.J., Frank, E.L., Rockwood, A.L. (2022). LC–MS/MS Method for High-Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine: Method for Analyzing Isomers Without Chromatographic Separation. In: Garg, U. (eds) Clinical Applications of Mass Spectrom...
Periodic acid-Schiff staining ELSA: Enzyme-linked immunosorbent assay Scr: Serum creatinine UPr: Urinary protein TNF-α: Tumor necrosis factor-α IL-6: Interleukin-6 PTH: Hormone GFR: Glomerular filtration rate ZO-1: Zonula occludens-1 MMP-2: Matrix metalloproteinase-2 α-SMA: ...
It is important to consider that most patients with MMA have low muscle mass and therefore the serum creatinine and calculated estimated glomerular filtration rate cannot accurately reflect true renal function. However, a study showed that cystatin C and serum methylmalonic acid concentrations were ...
To explore the dependence of glomerular filtration rate (GFR) on plasma total homocysteine (tHcy) and serum methylmalonic acid (MMA), as well as the consequences for the diagnosis of cobalamin and/or folic acid deficiency in an elderly community-dwelling population. Design and setting. Population-...
The questionnaire asked for clinical presentation and major complications (e.g. metabolic stroke, CRF, and pancreatitis), anthropometrics, blood pressure, routine laboratory tests (including complete blood cell count, serum creatinine, serum uric acid, and urinary MMA concentration), results of cranial...