MBDCap-Seq from formalin-fixed paraffin-embedded (FFPE) tissues provides equivalent methylation data to fresh-frozen DNA MDB proteins can discriminate between 5mC and 5hmC(Yong et al., 2016) Disadvantages: Base-pair resolution is lower (~150 bp), as opposed to single-base resolution with other...
In the present study, MethylCap-seq was used to characterize the genome-wide DNA methylation patterns in canine high-grade B-cell lymphoma (cHGBL). Canine methylated DNA fragments were captured and the MEDIUM-HIGH and LOW fraction of methylated DNA was obtained based on variation in CpG ...
Genome-wide DNA methylation detection by MethylCap-seq and Infinium HumanMethylation450 BeadChips:an independent large-scale comparison. T.De Meyer,P.Bady,G.Trooskens,S.Kurscheid,J.Bloch,J.M.Kros,J.A.Hainfellner,R.Stupp,M.Delorenzi,M.E.Hegi,W.Van Criekinge. Science Reports . 2015...
The workflow we describe performs MethylCap-seq experimental Quality Control (QC), sequence file processing and alignment, differential methylation analysis of multiple biological groups, hierarchical clustering, assessment of genome-wide methylation patterns, and preparation of files for data visualization. ...
First, the MethylCap-seq methodology was validated in zebra finch by comparison with RRBS-generated data. To assess the influence of (variable) methylation on gene expression, RNA-seq experiments were performed as well. Comparison of RNA-seq and MethylCap-seq results showed that at least 357 ...
MethylCap-seqCanine lymphomaMethylated markersCLBL-1The most common type of tumor occurring in canine is the lymphomas. Epigenetic factors like DNA methylation is one of the important factors for causing cancer in canine. Cytology-based screening methods, such as fine needle aspiration (FNA) or ...
PrEMeR-CG: inferring nucleotide level DNA methylation values from MethylCap-seq data.Motivation: DNA methylation is an epigenetic change occurring in genomic CpG sequences that contribute to the regulation of gene transcription both in normal and malignant cells. Next-generation sequencing has been ...
RNA- and MethylCap-seq experiments were performed on two zebra finch cell lines in presence or absence of 5-aza-2-deoxycytidine induced demethylation. First, the MethylCap-seq methodology was validated in zebra finch by comparison with RRBS-generated data. To assess the influence of (variable) ...
General characteristics of BCC-specific DNA methylation patterns determined by MBD methylCap-seq.doi:10.1371/JOURNAL.PONE.0035175.G001Zhao YangxingGuo ShichengSun JinfengH. ZhaohuiZhu Tong-yuZ. HongyuGu JunHe YinghuaWang WeiMa KelongPLOS ONE
MethylCap-seq is a robust procedure for genome-wide profiling of DNA methylation. The approach consists of the capture of methylated DNA using the MBD domain of MeCP2, and subsequent next-generation sequencing of eluted DNA. Elution of the captured methylated DNA is done in steps using a ...