BisulPlus™ Loci 5mc & 5hmC Detection PCR KitMethylation-Specific PCR Methylation-specific PCR (MS-PCR or MSP) is one of the most commonly used methods for gene/sequence-specific detection of DNA methylation. The DNA undergoes bisulfite conversion of cytosine to uracil and then the methylated...
The total turnaround time for analysis was <4 hours including DNA isolation, bisulfite conversion and qPCR analysis.The MMS-qPCR described here provides an accurate, accessible, and affordable liquid biopsy assay for the early detection of CRC. The same development strategy can also be applied to...
Methylation-Specific qPCR Methylation-specific quantitative PCR (e.g., EpiTect MethyLight PCR Kit, Qiagen) is a practical and popular strategy for gene-specific methylation analysis (Eads et al., 2000). This strategy employs the use of strategically designed sets of primer pairs for the same r...
METHODS: We used qPCR and pyrosequencing to investigate expression and epigenetic regulation of the calcium channel regulatory subunit a2d-3 (CACNA2D3) in breast cancer cell lines, primary cancers and metastatic lesions. RESULTS: Expression of CACNA2D3 mRNA is regulated in breast cancer cell ...
The expression levels of half of these genes were further validated by RT-qPCR (Fig. S17). Except for evm.model.Contig219_pilon.16 (psbA), the gene body regions of all other genes were associated with hypo-DMRs in the CG sequences context. Meanwhile, the upstream regions of five genes...
ACTIN2 served as the internal control in the RT-qPCR analysis. Error bars represent s.d. (n = 3 biologically independent samples). Full size image To clone the RDM15 gene, we used TAIL-PCR and found a T-DNA insertion in the 7th exon of AT4G31880 (Fig. 1c). A complementation...
CCND1 qPCR assay Conversion of 500 ng of RNA into cDNA was performed using the SuperScript II reverse transcriptase (Life Technologies) following the manufacturer’s protocol. Amplification was performed in duplicate with a technical replicate (n = 2 for cDNA conversion). PCR reactions consisted...
(Additional file5: Figure S4).AIM1was expressed in the heart, kidney and placenta, but showed minimal or no expression in the liver, lung and pancreas by qPCR analysis (Additional file6: Figure S5).AIM1was found to be bi-allelically expressed in the kidney and heart in two macaques (...
Plants adapted to diverse environments on Earth throughout their evolutionary history, and developed mechanisms to thrive in a variety of terrestrial habitats. When plants are grown in the novel environment of spaceflight aboard the International Space S
qPCREarly cancer detection requires identification of cellular changes resulting from oncogenesis. Abnormal DNA methylation patterns occurring early in tumor development have been widely identified as early biomarkers for multiple types of cancer tumors. Methylation-Specific PCR (MSP) has permitted highly ...