MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and cisplatin were obtained from Sigma Chemical Co, St. Louis, MO, USA. 5.4.1. Cell Cultures Human lung (A549), colon (LoVo and HCT-15) and pancreatic (BxPC3, PSN-1) carcinoma cell lines, along with melanoma (A...
The precipitant was separated by SDS-PAGE. The protein-compound 15 complexes were detected by the fluorescence of compound 15 (Ex: 505 nm, Em: 555 nm) (A). The gel was also stained by CBB (B). The display of “+” and “−” indicated the presence (+) or absence (−) of ...
Molecules 2015, 20, 21094–21102 On reaction with phosphorane generated in situ from methyltriphenyphosphonium bromide/t-BuOK, (+)-27 participated in isomerization at C-2 to give the thermodynamically more stable (+)-29 (ee = 90.6%), while under similar conditions (´)-28 yielded its ...