The methods to generate transgenic ani- mals and to control transgene expression. Biotechnology, 2002, 98(2-3): 145-160. DOIHoudebine LM (2002) The methods to generate transgenic animals and to control transgene expression. J Biotechnol 98(2–3):145–160...
By editing its genome, it can further be engineered to produce two enzymes, IaaM and IaaH, which can metabolize several intermediates to produce IAA, a hormone crucial for the regulation of developmental processes in the plants. The two genes encoding for IaaM and IaaH are introduced into the...
A transgenic animal with alterations in an H2-O gene is prepared by introduction of an altered H2-O gene into a host animal. The resulting transgenic animals produce a substantially greater frequency of high affinity antibodies compared to H2-O wild type animals. A method for the production of...
During early embryogenesis, animals pass through similar and characteristic stages of development1,2,3. First, during cleavage and blastula stages, embryos produce the building blocks of the future body plan through a series of cell divisions. Second, during gastrula stage, the cells are specified ...
In particular, a method based on the use of transgenic animals, in which the transgenic animals are tolerant of (tolerised) and measuring the immune response against specific antigens and then exposed to the antigen variants. 在一个实施方案中,所述转基因动物是人类II类MHC分子的转基因小鼠并且变异...
when these radicals are released in response to self-antigens, tissue damage is often the result. Inhibition of this process can be regarded as a measure for immunomodulating effects of compounds. The oxygen metabolites can produce light-emitting reactions (chemiluminescence), which is measurable if...
Transgenic animals as pharmacological tools This chapter concentrates on the technical aspects of establishing an animal model. The different methods have been outlined, references for detailed descriptions have been provided, and the potential application and the inherent weaknes... K Bürki,B Ledermann...
as well as transgenic animals in medical research, transgenic sheep and genetically modified micro-organisms in the food on the progress of the study, such as the application. 这篇文章描述transgenic kwa菜、米和其他可更新的系统,基因方法的创立,以及transgenic动物在医学研究, transgenic绵羊和基因上修改过...
leading to human lysozyme knock-in in approximately 1% of the treated bovine fetal fibroblasts. The milk secreted by the resulting transgenic cows could kill Staphylococcus aureus (S. aureus) [85]. The cell-free biosynthesis system is an efficient way to produce rare, easily degradable or toxic...
Scientists at the Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) have developed new methods to produce and analyze genetic mosaics.