The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the ...
Animals receive the test compound once a day for 5 days. Thereafter, they are sacrificed, spleens are removed, and a single cell suspension of 5 × 106cells/ml is prepared. Mitogens are titrated (four replicates/group) in 0.1 ml/well and 0.1 ml of the cell suspension is added. Pl...
A. User cloning and user fusion: the ideal cloning techniques for small and big laboratories. Methods Mol. Biol. 643, 185–200 (2010). Article CAS PubMed Google Scholar Reidhaarolson, J. F. & Sauer, R. T. Combinatorial cassette mutagenesis as a probe of the informational content of ...
Molecular cloning of ovine and bovine type I interferon receptor subunits from uteri, and endometrial expression of messenger ribonucleic acid for ovine re... Interferon-tau (IFN-tau), a type I IFN structurally related to IFN-alpha, is regarded as the major antiluteolytic factor secreted by th...
Metagenomics is a rapidly growing field of research that aims at studying uncultured organisms to understand the true diversity of microbes, their functions, cooperation and evolution, in environments such as soil, water, ancient remains of animals, or the digestive system of animals and humans. The...
Alexander LJ, Rohrer GA, Beattie CW (1996) Cloning and characterization of 414 polymorphic porcine microsatellites. Anim Genet 27:137–148 CASGoogle Scholar Allison NL, Destefano S (2006) Equipment and techniques for nocturnal wildlife studies. Wildl Soc Bull 34:1036–1044 ...
Molecular cloning and characterization of a novel protein of Trichinella pseudospiralis excretory-secretory products A novel excretory鈥搒ecretory (ES) protein ofTrichinella pseudospiraliswas produced. A cDNA library was constructed from mRNA of muscle larvae at 30 days p... I Nagano,Z Wu,T Nakada...
(in vector pTREtight2) was used as a template from which CACNA1E-eGFP was removed using restriction site AgeI, and fragments for FLAG-BK-YFP and LRRC8A-GFP was inserted, respectively, using Gibson cloning25,26. The FLAG-BK-YFP fragment was amplified from plasmid FLAG-BK667YFP27, a ...
SELECTION AND CLONING METHODS Selection and cloning methods are disclosed that are effective for the selective multiplication of desired animals, for instance livestock animals. These methods can be used to expand populations of animals, and are particularly useful f... PC Rebholtz,Amy K. Rebholtz...
Compared to traditional means, modern DNA assembly methods allow cloning of large, multigenic vectors for plant transformation in rapid fashion. These meth... DGJ Mann,SA Bevan,AJ Harvey,... 被引量: 0发表: 2019年 RNA interference: methods for plants and animals. The discovery that small RNA...