30 μg of protein lysates was incubated with 50 μmol/l of sphingosine (dissolved as 1 mmol/l stock solution in 4 mg/ml of BSA in PBS) and 10 μCi (370 kBq) of [γ-32P]ATP for 15 min at 37 °C. For SK-2 activity assay, the same buffer including 1 M KCl was used to ...
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The count table, a numeric matrix of genes × cells, is the basic input data structure in the analysis of single-cell RNA-sequencing data. A common preprocessing step is to adjust the counts for variable sampling efficiency and to transform them so
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Polygenic scores (PGS) can be used for risk stratification by quantifying individuals’ genetic predisposition to disease, and many potentially clinically useful applications have been proposed. Here, we review the latest potential benefits of PGS in the
2024, Talanta Citation Excerpt : This comprehensive range of methods plays a vital role in diagnosing viral infections, providing multiple methods for a variety of viruses and clinical situations. Several emerging virus detection methods include artificial intelligence [29–31] and machine learning [30...
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