是全球癌症相关死亡的主要原因之一,传统的二维(2D)细胞培养技术在研究肺癌的发生、发展和治疗中有一定的局限性。二维培养无法有效维持原代肿瘤细胞的长期增殖和分化能力,尤其是那些具有特定基因突变的肿瘤细胞(如Kras突变、EGFR突变等)。为克服这些限制,研究人员开发了三维(3D)空气-液体界面(ALI)培养系统,用于正常和癌变...
在分子生物学研究中,Methods Mol Biol缩写代表了《Methods in Molecular Biology》(分子生物学方法学)。这是一本重要的学术杂志,专门介绍各种在分子生物学领域中应用的方法和技术。这些方法和技术涵盖了从DNA和RNA分离、克隆、测序、基因编辑到蛋白质分析等众多领域,为生物学研究人员提供了最新的实验技术和理论指导。
January 01, 2024 [MEDLINE Abstract] Using Baker's Yeast to Determine Functions of Novel Wolbachia (and Other Prokaryotic) Effectors. January 01, 2024 [MEDLINE Abstract] Use of Drosophila Transgenics to Identify Functions for Symbiont Effectors. ...
Methods Mol Biol 下载文档 收藏 打印 转格式 146阅读文档大小:109.02K16页樂、白色上传于2016-01-06格式:PDF a short history and perspectives on plant genetic transformation.[2020][methods mol biol] 热度: metabolic control analysis for drug target prioritization in trypanosomatids.[2020][methods mol bi...
For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol ...
Methods Mol. Biol. 2075, 253–264 (2020). Article CAS PubMed Google Scholar Alanin, K. W. S. et al. An improved direct metamobilome approach increases the detection of larger-sized circular elements across kingdoms. Plasmid 115, 102576 (2021). Article CAS PubMed Google Scholar Kleme...
Methods Mol Biol. 2013;953:121–41. Article CAS Google Scholar Barkla BJ, Vera-Estrella R, Raymond C. Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. Bmc Plant...
SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation. Genome Biol. 19, 161 (2018). Article Google Scholar Lee, J., Hyeon, D. Y. & Hwang, D. Single-cell multiomics: technologies and data analysis methods. Exp. Mol. Med. 52,...
Cell Mol Biol. 2019;65(1):19–26. Article PubMed Google Scholar Yang Y, Huang XL, Jiang ZM, Li XF, Qi Y, Yu J, Yang XX, Zhang M. Quantification of chemical groups and quantitative HPLC fingerprint of poria cocos (Schw.) wolf. Molecules. 2022;27(19):6383: https://doi.org/...
Mol Biol Cell. 2006;17(8):3469–83. Article CAS PubMed PubMed Central Google Scholar Hsu C, Morohashi Y, Yoshimura S, Manrique-Hoyos N, Jung S, Lauterbach MA, et al. Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A-C. J Cell Biol. 2010;189(...