The present invention relates to a method for producing RNA. In particular, the present invention relates to a method for producing RNA, which is scalable and provides RNA at a high purity. The present invention provides a method for producing RNA under GMP and/or cGMP-compliant conditions. ...
Here, we describe a novel method for producing target cells that has provided satisfactory results in an F-CTL assay we have set up to assess envelope (Env)-specific CTL activity in domestic specific pathogen-free (SPF) cats infected with feline immunodeficiency virus (FIV), the feline equivale...
Producing PCR Products Guidelines for PCR Generally 10-100 ng of DNA is sufficient to use as a template for PCR. If amplifying a pool of cDNA, the amount needed will depend on the relative abundance of the message of interest in your mRNA populati...
Producing PCR Products Guidelines for PCR Generally 10-100 ng of DNA is sufficient to use as a template for PCR. If amplifying a pool of cDNA, the amount needed will depend on the relative abundance of the message of interest in your mRNA population. ...
In the CFPS step, we employed our techniques: 1) ‘Zipbody’ as a method for producing Fab, in which the association of heavy and light chains is facilitated by adhesive leucine zipper peptides fused at the C-termini of the Fab; and 2) an N-terminal SKIK peptide tag that can ...
The assay for transposase-accessible chromatin using sequencing (ATAC-seq) is widely used to identify regulatory regions throughout the genome. However, very few studies have been performed at the single cell level (scATAC-seq) due to technical challenges. Here we developed a simple and robust pl...
Construction of pDOC derivatives for generating lacI gene fusions Four different lacI gene fusions were constructed in MG1655, producing the following recombinant proteins; LacI::6 × His, LacI::3 × FLAG, LacI::4 × ProteinA and LacI::GFP. For the LacI::6 × His construct, two primers ...
c. separating polyadenylated mRNA substantially free of protein, DNA and other RNA, d. synthesizing a double-stranded cDNA wherein one strand has a nucleotide sequence complementary to that of the polyadenylated mRNA, thereby producing cDNA having a nucleotide sequence of a gene derived from a ...
This invention relates to a method for producing a recombinant viral expression vector. More particularly, this invention relates to a method for incorporating a selected gene coupled with a baculovirus promoter into a baculovirus genome to produce a recombinant baculovirus expression vector capable of ...
WO2007119574A2 2007-10-25 A METHOD FOR PRODUCING AN L-AMINO ACID USING BACTERIUM OF THEENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF A GENE CODING FOR SMALL RNAOther References: Chen et al. J. Bacteriol., vol. 186, No. 20, pp. 6689-6697 (2004). Kawamoto et al (Molecular Micr...