Miriam KahnF. A. AjmarOncologyBowman, J. E., Carson. P. E., Frischer, H., Kahn, M., and Ajmar, F. A.: A capillary tube, nile blue methemoglobin reduction test for glucose-6-phosphate dehydrogenase defi- ciency. Proceedings X International Con- gress Blood Transfusions, Stockholm,...
Each sample was divided equally among three test tubes. Tube 1 served as a negative control. Sodium nitrite .18 mol/L with dextrose .28 mol/L was added to tube 2 and to tube 3. Next, phosphate-buffered saline solution (PBS) was added to tube 2 and NAC (200 mg/mL) to tube 3. ...
(MHB) in an in-vitro model of glucose-6-phosphate dehydrogenase (G6PD) deficiency, given that methylene blue is an ineffective MHB antidote in G6PD deficiency.Methods: Five volunteers donated blood, which was divided equally into 2 test tubes, centrifuged, and washed with Tris-Mopps buffer (...
We found that the ability to detect visually an abnormal appearance of methemoglobin was significantly improved when the test sample was compared to control blood. The descriptive term "darker than normal" was much more commonly used than "chocolate" to describe the methemoglobin blood.Fred...