The IR-ECD spectrum of native [Aβ + 2H + Cu]4+ revealed that the [Arg5–Gln15] region—which includes His6, His13, and His14—contained the Cu2+ binding site (Figure 2b, d). Combined, these data suggest that the metal ion was coordinated by ligands in the N-terminal region, ...
DFT calculations using five different functionals were performed on the model complexes Cp3M−ECp (M = Nd, U; E = Al, Ga), and empirical fitting of the values for Cp3M−ECp allowed the prediction of binding energy estimates for Cp*Al compounds 1 and 3. NBO/NLMO bonding analyses on ...
Albumin and metal binding The NTS of albumin contains two metal ion binding sites. The first arises from the first triplet amino acid motif of albumin: Asp1–Ala2–His3 (the N terminal site)57. The second (also termed site B), is partially composed of His9 and Asp1358. While site B...
Even though coordination by a phosphate of AMP often creates a favorable cation binding environment, this site has an improbable geometry (tetrahedral) for magnesium (which prefers octahedral geometry) (a). The ion is better interpreted as a water molecule (b). The other two modeled magnesiums ...
Metal ions play critical roles in chemical, biological, and environmental processes. Various biomolecules have the ability to coordinate with metal ions and form various materials. Nucleobases, nucleosides, and nucleotides, as the essential components of DNA, have emerged as a useful building block for...
According to the characterization of the N–K-edge XAFS spectra, N-doping results in the binding energy of the N–NiO’ absorption edge changed to a smaller position, further leading a covalent effect. The existence of Ni–N band with the analysis of XPS confirmed the successful doping of ...
We have monitored the effect of salt on metal ion removal rate where no appreciable effect of salt (NaCl) on sorption was observed. Experiments at pH ∼ 6 shows that NPs were not able to pick up any of the studied metal ion but at pH ∼ 9, removal of Sr2+, La3+ and Th4+ ...
Tridentate binding units as structural patterns for the design of nine-coordinate lanthanide building blocks with predetermined properties an unprecedented fine tuning of the structural, thermodynamic and electronic properties of the lanthanide complexes, thus opening new perspectives for the ... Claude,Pigu...
T. Further characterization of the mouse sperm surface zona-binding site with galactosyltransferase activity. Mol. Reprod. Dev. 25, 393–399 (1990). Article CAS PubMed Google Scholar Wozniak, K. L. et al. Zinc protection of fertilized eggs is an ancient feature of sexual reproduction in ...
This protocol should allow experimenters to gather sufficient evidence to unambiguously determine the identity and location of the metal of interest, as well as to accurately characterize the coordinating ligands in the metal binding environment within the protein. Meticulous handling of metal-containing ...