In addition to the metalloenzymes with tightly bound metal retained during purification there are the related metal-activated enzymes1. The difference, however, is merely a quantitative one; the latter exhibit a lower affinity for the metal, so that the metal ion in general is lost during purif...
A large number of metalloenzymes and metal ion-activated enzymes are now known. Some metals, such as zinc and magnesium, are frequently associated with enzymes, although they may not be structurally a part of the molecule. A second major group of metal-dependent functions involves the ...
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Loosely bound (forming metal-activated enzymes) Tightly bound (forming metalloenzymes Coenzymes Tightly bound prosthetic groups 2 Loosely bound cosubstrates Many coenzymes are derived from vitamins . Table 1 lists vitamins, the coenzymes derived from them, the type of reactions in which they...
Metal-tolerant thermophiles are inhabitants of a wide range of extreme habitats like solfatara fields, hot springs, mud holes, hydrothermal vents oozing ou
Copper transporting P-type (P1B-1-) ATPases are essential for cellular homeostasis. Nonetheless, the E1-E1P-E2P-E2 states mechanism of P1B-1-ATPases remains poorly understood. In particular, the role of the intrinsic metal binding domains (MBDs) is enigm
Due to the growing prominence of artificial metalloenzymes and other peptide-based catalysts, reliable methodology for incorporating metal-binding functionalities into peptides is of great interest. Accordingly, numerous examples of unnatural amino acids with such functionalities have emerged in the ...
The vast difference in activities of ZAF(Ser) and ZAF was investigated in depth. It was speculated that, during the catalytic processes of artificial metalloenzymes, increased surface area or enhanced Lewis acidity at the metal active site would allow the artificial enzyme to accelerate hydrolysis ...
Maize (Zea mays L.) is a widely cultivated cereal and has been used as an optimum heavy metal phytoremediation crop. Metallothionein (MT) proteins are small, cysteine-rich, proteins that play important roles in plant growth and development, and the regul
Metal ions play critical roles in chemical, biological, and environmental processes. Various biomolecules have the ability to coordinate with metal ions an