靶向下一代测序(Targeted Next-Generation Sequencing,tNGS)是一种基于NGS的靶向富集测序技术,通过设计特异性引物或探针,对目标病原体或基因区域进行靶向扩增或捕获,然后进行高通量测序。这种方法能够显著提高目标病原体的检测灵敏度和准确性。 流程: 引物/探针设计:根据目标病原体或基因区域设计特异性引物或探针。 样本...
Metagenomic next-generation sequencing (mNGS) of circulating cell-free DNA from plasma is a hypothesis-independent broadband diagnostic method for identifi
Metagenomic next-generation sequencing (mNGS) is an unbiased approach to identify all the microbial genomes present in a clinical sample through brute-force sequencing and can detect viruses in parallel with other organism types [10]. From: Molecular Medical Microbiology (Third Edition), 2024...
We developed a metagenomic next-generation sequencing (mNGS) test using cell-free DNA from body fluids to identify pathogens. The performance of mNGS testing of 182 body fluids from 160 patients with acute illness was evaluated using two sequenc
Metagenomic next-generation sequencing of the CSF samples identified a pathogen in 20 (28.6%) subjects, which were also all identified by usual care testing. The median time to result was 38 hours. CONCLUSIONS. Metagenomic sequencing of CSF has the potential to rapidly identify pathogens in ...
Next-generation metagenomic sequencing (mNGS) has been applied in several scenarios: sepsis, meningitis and encephalitis, bloodstream infection, pneumonia, and others. In these situations, routine microbiologic testing is frequently insufficient to detect uncommon pathogens, making sequencing an attractive app...
This study aims to evaluate the diagnostic application and performance of the metagenomic next-generation sequencing (mNGS) in patients suspected of local pulmonary infection by comparing it to the traditional pathogen detection methods in lung tissue specimens obtained by a computerized tomography-guided ...
Hongxu Xu1, Min Liu1, Peisong Chen1* and Yili Chen1* Abstract Objective The aim of this study was to evaluate the effectiveness of metagenomic next-generation sequencing (mNGS) for the diagnosis of Pneumocystis jirovecii Pneumonia (PCP) in critically pediatric patients. ...
Next-generation sequencing (NGS) has been applied in the field of infectious diseases. Bronchoalveolar lavage fluid (BALF) is considered a sterile type of specimen that is suitable for detecting pathogens of respiratory infections. The aim of this study was to comprehensively identify causative pathoge...
Enhanced, unbiased diagnostic approaches are needed.To present a case series of patients with diagnostically challenging subacute or chronic meningitis using metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) supported by a statistical framework generated from mNGS of control samples...