The volume was increased to 20 µl, giving a final concentration of 0.25 nM each of dNTPs, 5 pmoles of oligo (dT)15/µl, 1 U/µl of RNase inhibitor, 200 U/ml of AMV Reverse Transcriptase XL (TAKARA Bio Inc., Japan). For the amplification of hgf gene, 3 µl of cDNA ...
Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, 1441 Eastlake Avenue, Suite 5410, Los Angeles, CA, 90033, USA Alberto Puccini, Heinz-Josef Lenz & Francesca Battaglin Medical Oncology Unit 1, IRCCS Ospedale Policlinico San...