项目名称 评价靶向间皮素(Mesothelin)的自体CAB-T细胞(PM3006注射液)在晚期实体瘤受试者中的耐受性、安全性及初步疗效的临床试验。 项目简介 CAB-T细胞免疫治疗在CAR-T的基础上研发,采集患者肿瘤组织内T细胞,体外编辑培养后回输。此外,CAB-T细胞还能激活体...
用于共同转录表达蛋白质的IRES核糖体结合序列;用于嵌合抗原受体基因的真核转录的人EF1α启动子;用于组成集识别,传递,启动于一体的二代CAR或三代CAR的嵌合抗原受体;用于增强转基因的表达效率的eWPRE元件.此外,还公开了该载体的构建方法和应用.本发明能显著提高细胞因子的分泌,CART细胞的体外杀伤作用,临床治疗恶性胸膜间...
CAR-T针对肿瘤实施精准靶向治疗,如果把靶向疗法看成“武警”在繁忙的“城市”里试图清除叛乱“暴徒”和他们的“据点”,那么,CAR-T就是“武警”,“暴徒”就是癌细胞,“据点”就是肿瘤所在的微环境,而“城市”就是我们的身体。首先,要想在繁忙的“城市”里找到“暴徒”,首先要识别这些“暴徒”,也就是我们说的...
Triple-negative breast cancer (TNBC) is an aggressive subtype with poor prognosis and limited treatment options. Chimeric antigen receptor (CAR)-T cell therapy holds promise, but its efficacy is hindered by tumor antigen escape and heterogeneity. To addr
Here, we have developed CAR-T cells that exploit the binding capacity of mesothelin to MUC16. We find that MSLN-CAR T cells effectively eliminate ovarian cancer tumor cells and stem-like cells in vitro, and this cytotoxic function is dependent on high expression levels of MUC16 on the tumor...
一提取的质粒、super piggybac transposase质粒与电转缓冲液混合,制得电转混合液,并用电转混合液转染pbmc细胞,得到转染后的pbmc细胞;步骤四:将转染后的pbmc细胞转入x-vivo培养基,用cd3/cd28磁珠激活扩增cd3阳性t细胞,用1μg/ml嘌呤霉素筛选并培养扩增转染后的t细胞,获得mesothelin嵌合抗原受体修饰的t细胞msln-car-t...
5.一种如权利要求1所述的靶向Mesothelin的复制缺陷性重组慢病毒的CAR-T转基因载体的构建方法,其特征在于,包括以下步骤:(1)将含氨苄青霉素抗性基因AmpR序列(如SEQ ID NO.1所示)、原核复制子pUC Ori序列(如SEQ ID NO.2所示)、病毒复制子SV40Ori序列(如SEQ ID NO.3所示)、用于慢病毒包装的慢病毒包装顺式元件...
A chimeric antigen receptor (CAR) T-cell therapy that targets the protein mesothelin showed no evidence of major toxicity and had antitumor activity in patients with malignant pleural disease from mesothelioma, according to results from a phase I clinica
Cancer Immunology, Immunotherapy (2023) 72:3401–3403 https://doi.org/10.1007/s00262-023-03510-7 CORRECTION Correction to: Anti‑mesothelin CAR‑T immunotherapy in patients with ovarian cancer Jiannan Chen1 · Jianhua Hu2 · Lili Gu1 · Feng Ji1 · Fan Zhang1...
实施例1:meso–cd19-car-tegfr基因序列的确定 从ncbi网站数据库搜索到抗meso抗体重链和轻链可变区基因序列信息(ss1),抗cd19抗体重链和轻链可变区基因序列信息(fmc63)序列在网站http://sg.idtdna.com/site上进行密码子优化,保证在编码氨基酸序列不变的情况下更适合人类细胞表达。