(A) preparation of the surface of the electrode, (B) bacteria stamps, (C) formation of the micro-contact imprinting (reproduced with permission from Ref. (Idil et al., 2017), © 2016 Elsevier). 4.1.2 Pseudomonas aeruginosa Pseudomonas aeruginosa (P. aeruginosa), the second pathogenic ...
number of protoplasts using a haemocytometer As a component of Murashige and Skoog basal salt media In the preparation of total ionic strength adjustment buffer (TISAB) Other Notes Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or ...
Preparation method The cost-effective preparation method of MES monohydrate, involves several key steps to ensure high production efficiency and excellent product quality. Firstly, an organic solution containing morpholine and sodium chloroethanesulfonate is mixed and heated under reflux at a temperature ran...
buffer substance MES Preparation and handling A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing ...
The enzyme assay was initiated by adding the assay solution followed by the substrate glucose-6-phosphate. After 15 min, the reaction was stopped by removing the assay solution and a new reaction cycle was initiated by the addition of fresh reaction buffer including the substrate. This step ...
This invention refers to the preparation of concoctions containing: proteolytic enzymes dissolved in a micro-emulsion, formed by lecithin, aliphatic hydrocarbon aliphatic alcohol, and a buffer solution with a pH ranging between 7 and 9, which corresponds to the pH region of optimum activity of the...
(i.e., organs) were exported to ZBrush for stylizing. Clear-cut artefacts (e.g., disruption of tissues during preparation) were removed and individual regions were highlighted and coloured in a knowledge-driven, uniform manner (i.e., stylized). After modelling, all models were re-imported ...
ignava allows for the preparation of a homogenous Mn2(IV,III) manganese population lacking traces of other EPR signals, which has previously not been observed for other class Id RNRs. This has allowed us to verify that the activity is not only highly dependent on the presence of ...
Renal tissue was obtained for further preparation. Control animals were injected isotonic saline into the tail vein and killed on days 2, 6, or 12 (ER4), or days 3, 5, 8, or 12 days (OX-7) after injection. Cell culture A clonal murine podocyte cell line carrying a temperature-...
PHD2 is essential in modulating HIF-1α levels upon oxygen fluctuations. Hypoxia, a hallmark of uterus, and HIF-1α have recently emerged as opposing regulators of mesendoderm specification, suggesting a role for PHD2 therein. We found that PHD2 expressi