doi:10.1016/S0076-6879(00)27289-3John D. FayenMark L. TykocinskiM. Edward MedofMethods EnzymolMethods in EnzymologyFayen, J. D. et al. Glycerolphosphoinositide anchors for membrane-tethering proteins. Methods Enzymol 327, 351-68 (2000)....
Tethering proteins greatly increase the volume of SNARE complexes and deform the site of hemifusion, which lowers the energy barrier for pore opening and provides the driving force. Thereby, tethering proteins assume a crucial mechanical role in the terminal stage of membrane fusion that is likely ...
An octameric protein complex involved in tethering secretory vesicles to the plasma membrane. Rab proteins Members of the Ras superfamily of small GTPases that are specifically involved in membrane trafficking events. Secondary ingression A constriction generated in the midbody that specifies the site ...
The functions of TMEM16 proteins are rather diverse based on the current knowledge of vertebrate TMEM16s and their homologues in lower organisms. A TMEM16 protein could be an intracellularmembrane proteinthat regulates organelle interactions, or serves as an ion channel, a lipid scramblase, or bot...
Early work in yeast and more recent studies in mammalian cells suggested that conventional membrane trafficking pathways control the fusion of autophagosomes with lysosomes; Rab GTPases are required to recruit tethering proteins which in turn coordinate the SNARE family of proteins that directly drive ...
Tied down: tethering redox proteins to the outer membrane in Neisseria and other genera Li X, Parker S, Deeudom M & Moir J (2011) Tied down: tethering redox proteins to the outer membrane in Neisseria and other genera. Biochemical ... L Xi,S Parker,M Deeudom,... - 《Biochemical ...
Rab8 and SNARE proteins interact with the Exocyst complex (eight proteins including Sec-6 and Sec-8) that aids in tethering of post-Golgi secretory vesicles to the plasma membrane. The vesicles are tethered at the BLM by the Exocyst complex prior to SNARE proteins coupling to and promoting ...
A Molecular Perspective on Mitochondrial Membrane Fusion: From the Key Players to Oligomerization and Tethering of MitofusinMitofusinFzo1Mitochondrial dynamicsMitochondrial fusionMitochondrial fissionDynamin-related proteinsMitochondria are dynamic organelles characterized by an ultrastructural organization which is ...
SNARE proteins were reconstituted by using the direct method described in ref. 13. Donor-dye and acceptor-dye proteoliposomes were reconstituted with autophagic t-SNAREs (STX17/SNAP29) and v-SNARE (VAMP8), respectively. SNAP29 and STX17 were mixed at a 1.5:1 molar ratio and incubated at ...
Structural basis for membrane tethering by a bacterial dynamin-like pairMicrobiologyStructural biologyDynamin-like proteins (DLPs) are large GTPases that restructure membrane. DLPs such as the mitofusins form heterotypic oligomers between isoform pairs that bridge and fuse opposing membranes. In ...