Cryo-electron microscopy structures of the human calcium-sensing receptor in complex with Giand Gqproteins reveal how this receptor activates distinct G protein subtypes and how its function is modulated by a variety of ligands. Feng He ,Cheng-Guo Wu ...
function, however, is still very sparse compared to that ofsoluble proteins(White, 2009). This sparseness is mainly due to the biochemical and biophysical technical difficulties associated with applying the available high-resolution techniques to membrane proteins. In approximately the past 35 years, ...
This asymmetry is created and maintained in vivo by dedicated biochemical pathways, but difficulties in creating stable asymmetric membranes in vitro have restricted our understanding of how bilayer asymmetry modulates the folding, stability and function of membrane proteins. In this study, we used ...
Proteins make up 50% of a plasma membrane’s mass. It occurs in two forms: peripheral (extrinsic) and integral (intrinsic). The membrane proteins for cell adhesion bind to the other proteins, such as those found in the extracellular matrix surrounding cells. Cell adhesion is hugely important...
Outer membrane proteins tend to be organized into trimers to allow their function as hydrophilic transmembrane channels. As such, the channel proteins are referred to as porins. Several porins may occur in one and the same cell and they have been studied in many Gram-negative organisms. A mono...
Proteins: Structure, Function, and GeneticsStevens, T.J. and Arkin, I.T. 1999. Are membrane proteins "inside-out" pro- teins? Proteins Struct. Func. Gen. 36: 135-143.Stevens TJ, Arkin IT. Are membrane proteins "inside-out" pro- teins? Proteins 1999;36:135-143....
membrane proteins function in complexes, which are a good proxy for interacting proteins. Moreover, we investigated this directly by evaluating the distribution of interaction degree normalized by the total number of interactions in datasets rich in membrane interactions (e.g., PF-PCA and SU-2HY,...
Chapter 11—Probes for Cytoskeletal Proteins Chapter 12—Probes for Organelles Chapter 13—Probes for Lipids and Membranes Chapter 14—Fluorescent Tracers of Cell Morphology and Fluid Flow Chapter 15—Assays for Cell Viability, Proliferation an...
Previous studies have established that the folding, structure and function of membrane proteins are influenced by their lipid environments1,2,3,4,5,6,7 and that lipids can bind to specific sites, for example, in potassium channels8. Fundamental questions remain however regarding the extent of mem...
1). Yet, the field of membrane protein structural biology is in a “log” phase. In recent years much effort has been put toward innovative developments to overcome the numerous obstacles associated with X-ray structure determination of membrane proteins. For instance much progress has been made...