产品特性 T-96 种类 倒顺式 额定电压 220 额定电流 24 接触电阻 20-39 绝缘电阻 20 加工定制 否 3C额定电压范围 400V及以下 3C证书编号 203925 可售卖地 全国 型号 52200024 概述: 20-39型倾斜开关/堵料开关垂直悬挂的倾斜开关在受到外力作用,偏离垂直位置15度以上时,开关内部的水银触点动作,常...
型号:Membrane Kit T-96(1盒4个膜头,10个O形圈,1瓶25ml电解液) 适用于:Inpro6050/12 Inpro6800/12 系列溶氧电极。 订货号:52200024 型号:Membrane Kit T-6900(1盒4个膜头,10个O形圈,2瓶5ml电解液) 适用于电极Inpro6900/12系列溶氧电极。
The experiments were carried out according to the instructions of Lysosome Isolation Kit (# LYSISO1, Sigma). Cells were pelleted and resuspended in 1× Exaction buffer and broken by Dounce homogenizer with Pestle B. The samples were then centrifuged at 1000 g for 10 min. The supernatants...
Proteins were then transferred onto PVDF membranes, blocked with 5% BSA in 1 × TBST and probed with the respective primary antibodies followed by the appropriate secondary antibody, and were subsequently visualised using an enhanced chemiluminescence kit (Thermo Scientific, US). Primary antibodies...
mRNA synthesis was carried out using the SP6 mMessage Machine kit (ThermoFisher #AM1340). Results Clinical presentation We report the index presentation of a 2-year-old female who presented with recurrent febrile urinary tract infections despite appropriate antibiotic prophylaxis. Other than nail ...
The gene was introduced into pQE30 by In-Fusion Cloning HD Kit (Takara Bio USA, Inc.). The primers used were 5′-GCATGCGAGCTCGGTGCTCCCAAAGAAATGCCAC-3′, 5′-AGGTCGACCCGGGGTCTACTTATAAGTAATCGAACC-3′, 5′- ACCCCGGGTCGACCTGCA-3′, and 5′-ACCGAGCTCGCATGCGGA-3′. Subsequently, ...
The crude membrane fractions were isolated using ProteoExtract Subcellular Proteome Extraction Kit (Merck KGaA, Darmstadt, Germany) according to the manufacturer's instructions. After measuring total protein concentrations in the crude membrane fraction by the Bio-Rad DC Protein Assay, the samples were ...
For determination of host cell growth, cell monolayers grown on 96-well plates for 48 h at 37 °C were examined using cell counting kit (Dojindo, Kumamoto, Japan). The number of host cells was assessed based on their dehydrogenase activities and examined as the absorbance (450 nm) of reduc...
Total RNA was isolated from HEK293T cells (ATCC) by TRIzol reagent (Invitrogen) according to the manufacturer’s instruction. cDNA library was obtained by using First Strand cDNA Synthesis Kit (Thermo Fisher). For FAF2-HA and CYB5R3-HA, the open reading frames without stop codon were PCR ...
A cell-based Cholesterol Assay Kit from Abcam (Cambridge, UK) was performed in intact C6 cells in order to visualize and measure cholesterol by using Filipin III as a fluorescence probe of cholesterol. Briefly, after removal of culture medium from wells, cells were fixed for 10 min and was...