FluidityFluorescence polarizationFatty acidsLDLLDL receptorCHOChinese hamster ovaryDPHdiphenylhexatrieneTMA-DPHDietary saturated fatty acids, especially lauric (12:0), myristic (14:0) and palmitic (16:0) acids,
highly sensitive to variations in the physicochemical properties of the cell membrane[162]. While higher membrane fluidity gives a more favorable environment for ABCA1 function, trans acyl chains in the membrane adopt extended configurations similar to saturated acyl chains[163], thus reducing fluidity...
Subcellular membranes have complex lipid and protein compositions, which give rise to organelle-specific membrane packing, fluidity, and permeability. Due to its exquisite solvent sensitivity, the lipophilic fluorescence dye Nile Red has been used extensively to study membrane packing and polarity. Further...
Ahydrophobicmolecule (or region of a molecule) repels and is repelled by water. Some lipid tails consist of saturated fatty acids and some contain unsaturated fatty acids. This combination adds to the fluidity of the tails that are constantly in motion. Phospholipids are thus amphipathic molecules...
In Regent, both DBI and unsaturated/saturated ratios increase at 6 and 12 hpi (Fig. 2D,E; Supplementary Fig. S2D,E). The ability to adjust membrane lipid fluidity by changing levels of unsaturated fatty acids is a feature of stress acclimating plants maintaining a suitable environment for ...
Fatty acids- As already mentioned, the cell membrane consists of both saturated and unsaturated fatty acids. Because of the single bonds between the carbon atoms of saturated fatty acids, they have been shown to be straight chains that tend to pack tightly. ...
we hypothesized that improving the membrane fluidity of host cells may be beneficial to β-carotene secretion. TheOLE1gene encoding stearoyl-CoA 9-desaturase catalyzes the transformation of saturated fatty acids (C16:0 and C18:0) to UFAs (C16:1 and C18:1) through a dehydrogenation reaction. Ov...
(saturated, monounsaturated and polyunsaturated) membrane fatty acid composition is homeostatically regulated despite diet variation. This is not the case for fatty acid composition of storage lipids, which responds to diet variation. Polyunsaturated fatty acids are important determinants of physical and ...
(1989a) Evidence for Critical Period Programming of Intestinal Transport Function: Variations in the Dietary Ratio of Polyunsaturated to Saturated Fatty Acids Alter Ontogeny of the Rat Intestine, Biochim. Biophys. Acta 1001, 302–315. Thomson, A.B.R., Keelan, M., Garg, M.L., and Clandin...
Methods for calculating average membrane lipid length and lipid saturated: unsaturated ratio can be found in [14]. Membrane Lipid Content Measurement: Thirty milliliters of mid-log E. coli cells were centrifuged, washed by ddH2O and adjusted to OD550˜10. Then, 1.8 ml of cell suspension ...