Cryo-electron microscopy structures of the ABCG2 protein in ATP- and substrate-bound states reveal the location of substrate binding, conformational changes required for substrate translocation and how inhibitors might be distinguished from substrates. Ioannis Manolaridis , Scott M. Jackson & Kaspar P...
Thus, immunization with GT5 can induce affinity maturation in rare PCT64 precursors and support the acquisition of PCT64-like mutations that neutralize autologous virus. mRNA-LNP membrane-bound GT5 trimers potently activate PCT64 precursors Nucleoside-modified mRNA vaccines for SARS-CoV-2 have ...
perforin. Both NK and NKT cells use the same mechanisms as the cytotoxic CD8+T cell for killing (i.e., perforin/granzyme cytotoxicity and Fas-ligand/fas cytotoxicity ) (seeFig. 84-7). The control and resolution ofviral infectionsrequire the elimination of the source of the virus and thus...
Beads were extensively washed with buffer A, and the bound protein was then eluted with buffer A complemented with 250 mM imidazole. To remove the N-terminal HisMBP tag, we added tobacco etch virus (TEV) protease to the eluted sample. The mixture was dialyzed overnight against buffer B ...
Influenza virus capture using membrane chromatography: Improving selectivity by matrix design and pseudo-affinity ligand interactionsMembrane chromatography is consistently used in the purification of viral particles like adenoviruses or influenza viruses. The lack of traditional diffusion-based limitations of ...
Advanced microscopy and biochemistry techniques facilitate the study of membrane domains; however, these domains still elude directin vivovisualization. The multiplicity of possible organizational states and their context-dependent nature most likely account for experimental inconsistencies. ...
Such growth factor independence has not been attributed to mutations in ligand receptor14,188 or receptor-associated signal transducer molecules.189 It is well known that erythroid cells in PV are hypersensitive to a variety of cytokines, including insulin-like growth factor (IGF-1),91 stem cell ...
They are all type I bitopic membrane receptors that uniformly incorporate a characteristic array of domains: they exhibit extracellularly a ligand-binding domain and between one and eight epidermal growth factor domains; intracellularly they exhibit one or two NPxY motifs that act both as ...
Other labels include ligands, which can serve as a specific binding pair member for a labeled ligand, and the like. A wide variety of labels routinely employed in immunoassays can readily be employed in the present assay. The choice of the label will be governed by the effect of the label...
The SpyTag has covalently bound to a chimera of the SpyCatcher and a cargo protein (model structure of SP1690) through the formation of an isopeptide bond between the SpyTag and the SpyCatcher. The protein structures were generated using PyMOL. FIG. 2. Ligation of OMVs displaying SpyTag using...