Like other PB1 domains, the MEKK3PB1 adopts a ubiquitin-like fold consisting of a five-stranded β-sheet, an α-helix inserted between strands β2 and β3, and a second helix between β4 and β5 (ref. 14). MEKK3 grasps the CCM2HHD, with the thumb (MEKK3N) resting in the ...
Proteins pulled down with glutathione-Sepharose 4B resin were subjected to SDS-PAGE and stained with Coomassie Brilliant Blue. Interaction Surface of aPKC PB1 as a Type I PB1 Domain— The electrostatic surface potential of PKCι PB1 is shown in Fig. 5A. The conserved acidic residues in the...
Like other PB1 domains, the MEKK3PB1 adopts a ubiquitin-like fold consisting of a five-stranded b-sheet, an a-helix inserted between strands b2 and b3, and a second helix between b4 and b5 (ref. 14). MEKK3 grasps the CCM2HHD, with the thumb (MEKK3N) resting in the groove ...