free energy value was−21.0kcal/mol, which is within the range of genuine mirna-target pairs. additionally, there was perfect base-pairing between the seed region and the cognate target, which means that the mir-181a-5p binding sequence in the meg2 3′-utr is highly conserved among ...
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Briefly, serum-free and 10% serum-containing medium were added to the upper and lower layers in a 24-well Transwell chamber with a pore size of 8 μm. Cells were cultured in the upper layer for 24 h. Cells penetrating to the lower layer were subsequently fixed with 4% methanol and ...
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Otobius megnini, the “spinose ear tick,” has a broad host range, including ungulates,sheep,goats, cattle, horses,dogs, and human beings. Adults are free living and nonparasitic, but nymph and larval stages are parasitic. Newly hatched larvae remain in the environment until they climb onto...