" Medium-Chain Acyl-CoA Dehydrogenase- and Enoyl-CoA Hydratase-Dependent Bioactivation of 5,6-Dichloro-4-thia-5-hexenoyl-CoA " Biochemistry 1995 vol. 34: 4276-4286.Fitzsimmons, M.J., Thorpe, C. & Anders, M.W. (1995) Medium- chain acyl-CoA dehydrogenase- and enoyl-CoA hydratase- ...
3.3.22,3 enoyl-CoA hydratases 3.3.2.1Rationale Twohydrataseenzymesare thought to be responsible for the complete oxidation of long-chain fatty acids. Long-chain enoyl-CoA hydratase (LHYD) is part of the inner-mitochondrial trifunctional protein (TFP), and can be assayed usingultravi...
FadB, the dual-function enoyl-CoA hydratase, and dehydrogenase, can isomerize (R)-3-hydroxyacyl-CoA via the corresponding enoyl-acyl-CoA, likely at a reduced rate relative to its regular substrate isomer40,41,42,43. PHA-producing bacteria solve this problem by expressing an alternative enoyl...
Deficiency of all three TFP enzymes (LCHAD, long-chain enoyl-CoA hydratase, and 3-ketoacyl-CoA thiolase) is caused by almost all other variants of either the HADHA or HADHB genes. TFP deficiency is considered extremely rare. Our review supported this finding with an incidence of 0.13 per 100...
BIOSYNTHESIS OF MEDIUM CHAIN LENGTH POLYHYDROXYALK 优质文献 相似文献 参考文献 引证文献Fukui, T., Yokomizo, S., Kobayashi, G. & Doi, Y. Co-expression of polyhydroxyalkanoate synthase and (R)-enoyl-CoA hydratase genes of Aeromonas caviae establishes copolyester biosynthesis pathway in Escherichia ...
Enoyl-CoA hydrataseMedium-chain fatty acids are molecules with applications in different industries and with growing demand. However, the current methods for their extraction are not environmentally sustainable. The reverse 尾-oxidation pathway is an energy-efficient pathway that produces medium-chain ...
Park SJ & Lee SY ( 2003 ) Identification and characterization of a new enoyl coenzyme A hydratase involved in biosynthesis of medium-chain-length polyhydroxyalkanoates in recombinant Escherichia coli . J Bacteriol 185 : 5391 – 5397 .Park, S.J., Lee, S.Y. "Identification and ...
Our approach involved peroxisomal targeting of a 3-ketothiolase, acetoacetyl-CoA reductase, enoyl-CoA hydratase and PHA synthase, as well as plastid targeting of a acyl-ACP thioesterase and 3-ketoacyl-ACP synthase to increase peroxisomal 尾-oxidation flux. Of 143 transgenic sugarcane lines ...
Core genes for r-BOX such as thiolase, hydroxyacyl-CoA dehydrogenase, enoyl-CoA hydratase, and enoyl-CoA reductase were chromosomally overexpressed under the control of a cumate inducible phage promoter. Native E. coli thioesterase YdiI was used as the cycle-terminating enzyme, as it was found ...
eutropha (PhaCRe), Aeromonas hydrophila (PhaCAh), and Pseudomonas putida (PhaC2Pp) and with (R)-specific enoyl-CoA hydratases from P. putida (PhaJ1Pp and PhaJ4Pp), and A. hydrophila (PhaJAh). When glycerol supplemented with dodecanoate was used as primary carbon source, E. coli ...