For quantitative mRNA analysis, a template equivalent to 20 ng of total RNA was subjected to 40 cycles of quantitative PCR using the Takara SYBR Premix Ex TaqTM (TliRNaseH Plus, Takara,Tokyo, Japan) in the 7900HT Fast Real- Time PCR System. GAPDH was used for normalization. Relative mRNA...
the inclusion of communication-related topics at training seminars (Bangkok, Riga, Dar es Salaam cluster), the distribution of the CD-ROM for National Commissions, a new brochure on “SkillsforHandlingtheTV Media” and the creation of a website template for National Commissions, the publication ...
Germany). cDNA synthesis was performed with Bio-Rad iScripTM cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) according to the manufacturer's instructions in a reaction volume of 20 μl. For quantitative mRNA analysis, a template equivalent to 20 ng of total RNA was subjected to 40 c...
Germany). cDNA synthesis was performed with Bio-Rad iScripTM cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) according to the manufacturer's instructions in a reaction volume of 20 μl. For quantitative mRNA analysis, a template equivalent to 20 ng of total RNA was subjected to 40 c...
Change is unlikely, given industry pressure to template risk information combined with a shortage of evidence about what works best and when. The relative merits of quantitative and qualitative descriptors of risk is problematic. One study has argued that quantitative descriptors of risk are necessary...