The molecular weight of GajA tetramer was about 276 kDa. The retention volume of rabbit aldolase (158 kDa) is indicated by the dotted line. c, The protein purity was visualized by SDS-PAGE followed by Coomassie-blue staining. Images are representative of three biological replicates. d, ...
The reaction mixture was first separated by SDS-PAGE (lower panel), blotted, and developed with anti-ADPR reagent in western blot (upper panel). Auto-ADP-ribosylation of Ia is marked with an asterisk. The experiment was performed twice, a representative result is shown. b The fractions of ...
Total protein was quantified using the BCA Protein Assay Kit (GBCBIO, Guangzhou, China), and equal amounts of protein were separated by SDS-PAGE and transferred to PVDF membranes. Membranes were blocked with skimmed milk and incubated with primary and secondary antibodies (Additional file 1: ...
Yang Xin Tang (YXT) is a traditional Chinese herbal preparation which has been reported to improve cognitive function and memory in patients with dementia. As the underlying mechanism of action of YXT has not been elucidated, we examined the effects of Y
6-fucosyltransferase GDP, guanosine 5′-diphosphate GMP, guanosine 5′-monophosphate GTP, guanosine 5′-triphosphate HPLC, high-performance liquid chromatography IDP, inosine 5′-diphosphate PPi, pyrophosphate SDS–PAGE, sodium dodecylsulfate–polyacrylamide gel electrophoresis XDP, xanthosine 5′-...
Sequence analysis of SDS22 revealed that it is a leucine-rich repeat (LRR) protein, suggesting that SDS22, unlike nearly every other known PP1 regulator, is not an IDP but instead is fully structured. Here, the 2.9 resolution crystal structure of human SDS22 in space...
The m35 mRNA expression of strains 300, O35E and 415 after exposure to amoxicillin and different stress conditions was measured by real-time PCR and normalized in relation to their 16S rRNA expression. The expression of M35 protein was analysed by SDS-PAGE and western blotting. RESULTS: ...
c, Coomassie stained SDS-PAGE of the pooled protein sample visualized by cryo-EM showing the presence of the N-terminal ECR and the 7TM domain. d, Anti-FLAG Western-blot analysis of the LPHN3 sample purified in detergent. e, Cryo-EM reference-free 2D class averages of LPHN3 purified ...
Purity of the complex protein was monitored at all stages of the purification process using SDS-PAGE. The reRNA and DNA were monitored using 10% TBE-urea-PAGE gel electrophoresis and visualized by EB staining. Purified complexes were concentrated to 10–15 mg/mL for cryo-EM analysis. The ...
20 to 25,000 on non-reduced SDS-PAGE, is sensitive to protease K, and augments the effect of FAA on endothelial-cell-tissue factor. This activity is not found in supernatants from non-neoplastically transformed cell lines. These data lead us to hypothesize that FAA exerts its action, at ...