Proc.Natl.Acad.Sci.USA Vol.96,pp.9954–9959,August1999 PlantBiology ProtochlorophyllideoxidoreductaseB-catalyzedprotochlorophyllide photoreductioninvitro:Insightintothemechanismof chlorophyllformationinlight-adaptedplants NIKOLAILEBEDEVANDMICHAELP.TIMKO* DepartmentofBiology,UniversityofVirginia,Charlottesville,VA22903 ...
Mechanism and thermochemistry of oxidation of HCl and HBr at high temperatures. The heat of formation of HO2Using currently available thermochemical and kinetic data and estimation methods to analyze the thermochemistry and the kinetic parameters of the elementary reactions involved in the oxidation of ...
In addition, the cell surface of Ochrobactrum anthropi could accumulate more chromium in Tris-HCl buffer solution than in LB medium (Li et al., 2008), because the small-molecule organic compounds in LB medium could compete with the cell debris for Cr(III) coordination. In this work, the ...
The pH values of the Cr(VI) solution were adjusted to 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 and 7.0 with 0.1 mol L−1 HCl and NaOH solution, respectively. 100 mL Cr(VI) solutions with different pH values were placed in triangular bottles, and EW-BC film was added at the rate ...
Cell pellets (∼150 g) were harvested by centrifugation, washed with water and lysis buffer (50 mM Tris.HCl [pH7.4], 200 mM NaCl, 1 mM EDTA). Cells were resuspended in 100 mL of lysis buffer with 1 mM phenylmethylsulfonylfluoride (PMSF) (Roche) and 1.5 μM of ...
Kinetics of Thermal Growth of HCl-0[sub 2] Oxides on Silicon Thermogravimetric Analysis (TGA) techniques provide an accurate laboratory method for the study of oxidation and corrosion of engineering materials in aggr... K Hirabayashi,J Iwamura - 《Journal of the Electrochemical Society》 被引量:...
on the imidazolium ring and [Al 2 Cl 7 ].The alkylation reaction is initiated by the Br nsted acid [AlHCl 3 ] + which reacts with 1-dodecene to form a carbonium ion,then the carbonium ion reacts with benzene to form an unstable σ complex,leading to the formation of 2-phenyl...
The reaction mixture was diluted with diethyl ether and washed sequentially with 1M aqueous HCl solution, saturated aqueous NaHCO3 solution, water and brine. The organic phase was dried over MgSO4 and the solvent was removed under reduced pressure. Purification by flash column chromatography on ...
Quantification of mtDNA copy number Total DNA was extracted from liver tissues as previously reported21. Briefly, DNA was extracted using lysis buffer (10 mM Tris–HCl, pH 8.0, 150 mM NaCl, 20 mM EDTA, 1% SDS, and 0.2 mg/ml Proteinase K) and incubated at 55 °C for 6 h. After extr...
All ammonium sulfate buffers contained 100 mM Tris HCl pH 7.5 and 10 mM EDTA. In vitro replication assays For MCM loading and phosphorylation, 3 nM plasmid DNA template was incubated with 5 mM ATP, 75 nM Cdt1/Mcm2-7, 45 nM Cdc6, 20 nM ORC, 50 nM DDK in 25 ...