ILC2s were cultured in Roswell Park Memorial Institute 1640 medium (RPMI 1640, VivaCell) supplemented with 10% FCS, 100 U/mL penicillin/streptomycin, 1 mM sodium pyruvate, and 1× non-essential amino acids, plus 10 ng/mL IL-2 (Novoprotein) and 10 ng/mL IL-7 (Novoprotein). Fo...
The cultures were diluted back to about 30% confluence, and then an aliquot of cells was further diluted by at least 1/10–1/30 before plating in RPMI (Roswell Park Memorial Institute) medium with 5% serum and antibiotic/antimycotic at 1x. Cells usually attached within several hours, but ...