巴氏灭菌骆驼乳中耐甲氧西林(mecA基因)和耐热金黄色葡萄球菌菌株的患病率 食源性病原菌包括许多可能引发食物中毒的细菌,例如金黄色葡萄球菌(Staphylococcus aureus)在全球范围内是导致机会性、医院和社区相关疾病暴发的主要原因。 金黄色葡萄球...
PCR mecA geneMethicillin resistant Staphylococcus aureusPCR目的 應用mecA基因PCR擴增法檢測耐甲氧西林金黃色葡萄球菌(methicillin resistant staphylococcus aureus, MRSA).方法 臨床分離的70株金黃色葡萄球菌,應用mecA基因PCR擴增法鑒定MRSA,並與苯唑西林紙片擴散法進行比較.結果 70株金黃色葡萄球菌用PCR擴增法和紙片擴散...
根据释放的g-quadruplex/nmm复合物浓度与荧光强度成线性关系(λex=399nm,λem=610nm),从而达到检测金黄色葡萄球菌meca基因的目的。因为mecagene是金黄色葡萄球菌的一段致病基因,只存在于金黄色葡萄球菌中,所以可以通过检测mecagene达到检测金黄色葡萄球菌的目的。 在最佳条件下,该方法的线性范围从10fm到10nm,检测限为...
名称编号规格价格手册 Staphylococcus aureus金黄色葡萄球菌mecA基因染料法qPCR试剂盒14-32110-mecA50T2990元 英文名称:StaphylococcusaureusmecA SYBR PCR Kit 运输:Cold 保存:-20℃ 有效期:1 year 货期:2-3 Week 其他: 产品介绍: 14-32110-mecA 金黄色葡萄球菌mecA基因探针法PCR试剂盒使用手册V1.0(1) ...
aureus, because mecA is expressed by other methicillin-resistant bacteria. Biotium also offers the Staphylococcus aureus (nuc) PMA-PCR kit , which includes primers against the S. aureus-specific nuc gene, expressed by methicillin-susceptible and methicillin-resistant S. aureus strains. Viability ...
The evolution of Staphylococcus aureus Ruud H. Deurenberg, Ellen E. Stobberingh, in Infection, Genetics and Evolution, 2008 The mecA gene, which is 2.1 kb in length, is located on a mobile genomic island, that is called staphylococcal cassette chromosome mec (SCCmec) (Ito et al., 2003)...
1. A detection method employing a RNA amplification process, which comprises the steps of: forming a cDNA with a RNA-dependent DNA polymerase using a specific sequence of a RNA derived from mecA gene, a gene element of methicillin-resistant Staphylococcus aureus, present in a sample as a ...
塑璺—— ofmecAin aureusanditseffect DistributionStaphyIococcus onresistancetoantibiotics Abstract of veInordertounderstandthemechanism aureus(S. Obiecti Staphylococcus toantibioticsandtheroleofmecA structural aureus)resistance gene,the of protein2a, inthemechanism. genepenicillin...
antibioticstoStaphylococcus.The DNAofthesestrains weregotandpurified.Polymerase chainreactionswerec。nductedtoampify themecA gene.Thenanalyse therelationbetweenthemecA gene andthe resistanceOfS.aureus. Resu1ts ①Of 47strains,33(70.2%)wereMRsA.Of33MRsA,only3strains weresusceDtibletoantibioticsotherthanGlycop...
实时pcr装置使用了rotor-geneqmdx5plexhrm(qiagen)。pcr的反应条件为:于95℃加热5分钟后,将94℃10秒、65℃10秒、72℃30秒重复40次。将在三重测定中都能观察到扩增的最低浓度作为检测限。需要说明的是,通过tm值的测定确认是否得到了目标扩增产物。结果,筛选了较之使用作为在先申请的专利文献1中示出的比较用引物...