Detection of methicillin-resistance (mec-A) gene in Staphylococcus aureus strains by PCR and determination of antibiotic susceptibility. Ann Microbiol 2007;57:273-6.Zamani AR, Sadaghian S, Najafiemosleh M, Goudarzi MT, Yoysefi- Moshouf R, Ghaderkhani J. (2007) Detection of methicillin-...
Resistance to oxacillin is mostly mediated by the mecA gene, which codes for the production of a supplemental penicillin-binding protein, PBP2a or 2′, which is expressed either homogeneously or heterogeneously4,5. Expression of resistance in some MRSA strains is also regulated by homologues of th...
These species are often associated with infections in immunocompromised patients who have a medical device implant. Methicillin resistance was first described in Staphylococcus aureus; it has also been reported in CoNS species. Methicillin resistance is conferred by expression of the mecA gene, contained...
35引起细菌细胞壁合成破坏导致细菌死亡。但是MRSA由于有新的PBP2a内酰胺类抗菌药物不能与之结合细菌多肽层合成不受影响。MecI和MecR1主要调节mecA基因转录。在无β内酰胺类抗菌药物存在时MecI可以抑制mecA和mecR1mecI的转录。当β内酰胺类抗菌药物存在时mecR1被自动催化剪切
resistance, mediated by mecA gene encoded low affinity penicillin-binding protein (PBP) 2a, has been observed in high proportion of CoNS isolates. Accurate detection of methicillin resistance among CoNS isolates is an important factor in guiding antibiotic therapy. The mecA gene is a part of the ...
[4] 4. The method of claim 3 in which the control cell is a normal cell. [5] 5. The method of claim 3 in which the control cell is a tumor cell. [6] 6. The method of claim 3 in which the control cell has a mutated MEC1 gene or an analog or homolog thereof. ...
Similarly, dun1Δ cells exhibit sensitivity to AZC and Htt103Q and resistance to CHX; however, unlike mec1-4, dun1Δ cells are resistant to heat. rad53-K277A, a kinase dead allele impaired in the DDR, differs from both mec1-4 and dun1Δ in that it confers acute sensitivity to CHX...
因均位于此处,因此,也有人将其称为金黄色葡萄球菌的耐药岛(ResistanceIsland,RI)1～3。 SCCmec可自发剔除、重组的特性和耐药岛功能,使它成为了耐药基因传递的运载工具和储存池, 是耐药性产生、耐药谱扩大的根本原因。为了研究 MRSA的起源、流行、多重耐药性扩大及传递机制, ...
裂解液A、裂解液B及mecA、sasX、psm-mec、pvl基因PCR扩增试剂盒、相关阳性DNA对照均由无锡市克隆遗传技术研究所提供。 1.3 药物敏感性试验 用K-B纸片扩散法测定红霉素(ERY)、庆大霉素(GEN)、阿米卡星(AMK)、克林霉素(CLI)、复方磺胺甲噁唑(SXT)、万古霉素(VAN)、利奈唑胺(LZD)7种抗菌药物的敏感性,质控菌株...
Intracellular accumulation of abnormally phosphorylated tau in different types of neurons is a pathological characteristic of Alzheimer’s disease (AD). While tau modification and associated neuronal loss and hypometabolism start in the entorhinal cortex