Tuna meat samples (10 g) from the HPP treatment and control groups were added to sterile saline solution (90 mL), followed by homogenization at 3000 rpm for 120 s using a sterilized homogenizer (Omni International, Waterbury, CT, USA) and 10-fold serial dilution using sterile saline. The ...
The sample was concentrated in Uriprep (Waterbury, CT, USA), aliquoted in a 50 mL volumetric flask, centrifuged, and filtered with a 0.2 μm syringe filter. After about 40-fold dilution with lithium diluent (pH 2.36), 10 μL of the sample was injected into the Agilent 1100 Series (...