Enzymatic and mechanical tissue dissociation was performed using a gentleMACS skeletal muscle tissue dissociation kit (Miltenyi Biotec, Auburn, CA). A short pre-plating step of 25 min on uncoated plastic culture flasks was used to remove many of the rapidly adhering non-MPC cell types in order...
The mixture was vortexed, heated in a water bath at 80 °C for 20 min, cooled in cold water for 10 min, centrifuged at 4000 ×g for 20 min and filtered through a 0.45 μm hydrophilic polytetrafluoroethylene 13 mm syringe filter (Membrane Solutions, LLC, Auburn, WA, USA). The ...
(2007). Feed was removed 12 h before processing, after which preselected birds were placed in coops and transported to the Auburn University Processing Plant. Broilers were electrically stunned, exsanguinated, scalded, mechanically picked, mechanically eviscerated, and then placed on ice. Whole ...
After the 60-day feeding period, goats were transported to Auburn University for harvest. Goats were harvested at the Lambert-Powell Meats Laboratory using USDA humane slaughter standards under simulated commercial conditions. Carcasses were chilled after harvest procedures at 2.0 ± 1.0 °C for 24 ...