Advantages and disadvantages of different methods are compared. Importance of the relationships with functionality of proteins in food processing is emphasized because the true meaning of surface hydrophobicity of proteins is difficult to define. Keywords: probe spectrofluorometry; detergent binding; ...
Theoretical and Experimental Evaluation of Hydrophobicity of Proteins to Predict their Partitioning Behavior in Aqueous Two Phase Systems: A Review Hydrophobicity is the main determinant in the partitioning of proteins in Aqueous Two-Phase Systems (ATPSs). There are a range of different methods availab...
Considering the high content of protein and essential elements of edible mushrooms (cultivated and wild), its daily consumption in the world is increasing. Furthermore, wild and farmed mushrooms have been partially substituted for animal proteins in many countries, especially in Europe and Asia, due...
Ultrafiltration, protein precipitation and solid-phase extraction were investigated for purification of blood samples and enrichment of various compounds with a wide range of hydrophilicity and hydrophobicity. Protein precipitation by methanol and perchloric acid is a fast method to separate the analytes ...
Subtle changes in fusion activities of GP64 proteins containing single amino acid substitutions in a putative membrane fusion domain were distinguished, and decreases in EGFP fluorescence corresponded to decreases in the hydrophobicity in the small putative membrane fusion domain. 展开 ...
PROTEIN MEASUREMENT IN BIOPHARMACEUTICALS 1. INTRODUCTION AND SCOPE Many medicinal products are produced through recombinant technology via a host cell (e.g., bacteria, yeast, or mammalian, insect, or plant cell lines). During the manufacture of such products, some amount of non-product, host ...
and hydrophobicity, and hcps display a wide range of molecular weights (from ~5 kda to at least ~250 kda), depending on the host cell and manufacturing process used. the number of hcps in upstream samples can run anywhere from several hundred to more than one thousand proteins, depending ...
The pH sensitivity of hydrogel displacement can also be controlled by varying the elements of the polymer composition, such as the pKa of ionizable pendant groups, the amount of ionizable pendant group, crosslinking density, crosslinker type, and hydrophobicity of the polymer backbone. The glucose...
Recently graphene has been shown to turn hydrophilic when floating on water due to the wetting transparency effect10,36. In order to test the effect of the environment on one side of graphene on its hydrophobicity on the other side, we performed the experiments under different humidities. We ...
They can also be used to monitor the unfolding of protein molecules as the latter are pulled from the sample surface by the AFM tip. In the diagram of Figure 5 is shown a typical F vs. D curve obtained with a soft cantilever on a hard sample. Segment a–d represents the first half ...