本发明公开了一种MDCK‑MDR1的药物吸收筛选体系及其构建方法,针对MDCK细胞中犬P‑gp基因设计出了sgRNA,将设计的sgRNA序列合成Oligo,构建sgRNA表达载体;将人源MDR1基因转染至MDCK‑KO细胞中,筛选扩大阳性单克隆;蛋白免疫印迹法检测p‑gp蛋白表达,成功表达者即表示MDCK‑MDR1的药物吸收筛选体系构建成功。本发明...
If the K I is defined by the free energy of binding to P-gp, which kinetic parameters define the IC50 for the MDCKII-hMDR1 confluent cell monolayer?Annie AlbinPoulomi AcharyaJoseph W PolliJoe Bentz