因此,作者明确MdPH5是苹果中负责苹果酸转运和液泡酸化的关键P-ATPase。 图3 MdMYB73结合MdPH5基因启动子并激活MdPH5表达 为解析MdPH5介导的苹果酸转运和液泡酸化的分子调控机制,作者利用酵母单杂交筛库实验筛选到一个MYB家族转录因子MdMYB73;并进一步利用凝胶迁移实验(EMSA)、染色质免疫共沉淀PCR实验(ChIP-PCR)和双...
Functional analysis showed that Md MYB73 significantly increased cold tolerance in transgenic apple calli and Arabidopsis. Quantitative real-time PCR(q RT-PCR) assay indicated that the expression levels of cold-responsive genes including Md CBF2,Md CBF3,Md CBF4,and Md CBF5 were obviously enhanced...
vacuolaracidificationinappleKeywords:apple,R2R3-MYBtranscriptionfactor,MdMYB73,malateaccumulation,vacuolarpH,MalusdomesticaBorkh..SUMMARYMalate,thepredominantorganicacidinmanyfruits,isacrucialcomponentoffruitorganolepticquality,includingtasteandflavor.Thegeneticandenvironmentalmechanismsaffectingmalatemetabolisminfruitcellshave...
aMdBT2 degraded MdMYB73 in vitro. Cell-free degradation assays were performed to examine the stability of the MdMYB73 protein. Three types of apple calli (WT, 35S::MdBT2, and 35S::anti-MdBT2) were coincubated with MdMYB73-HIS protein at 22 °C for the times indicated.bDegradation ...
Interestingly, we isolated and identified a MYB transcription factor (TF), MdMYB73, a distant relative of MdMYB1 in this study. It was subsequently found that MdMYB73 protein bound directly to the promoters of MdALMT9 (Aluminum-activated Malate Transporter 9), MdVHA-A (Vacuolar ATPase ...
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How can I check my system's hardware? Click the Start button and type "system information" then open it. You can find most of the details you need on the first page, in the System Summary node. You can also expand each node in the left pane for additional information. ...
为解析MdPH5介导的苹果酸转运和液泡酸化的分子调控机制,作者利用酵母单杂交筛库实验筛选到一个MYB家族转录因子MdMYB73;并进一步利用凝胶迁移实验(EMSA)、染色质免疫共沉淀PCR实验(ChIP-PCR)和双荧光素酶实验(LUC)证明,MdMYB73直接结合MdPH5基因启动子并激活MdPH5表达。作者利用苹果果实瞬时注射实验,验证了MdMYB73作用...
为解析MdPH5介导的苹果酸转运和液泡酸化的分子调控机制,作者利用酵母单杂交筛库实验筛选到一个MYB家族转录因子MdMYB73;并进一步利用凝胶迁移实验(EMSA)、染色质免疫共沉淀PCR实验(ChIP-PCR)和双荧光素酶实验(LUC)证明,MdMYB73直接结合MdPH5基因启动子并激活MdPH5表达。作者利用苹果果实瞬时注射实验,验证了MdMYB73作用...