mda-mb-231 Cells Organism TypeHumanHuman Breast Cancer Cell Line ATCC HTB-26 TissuesBreast/Mammary PhenotypeAdherent Primaryno ApplicationCell Culture / Growth Conditions,Transient Transfection Every step of the way, a wide range of cell health products...
Malondialdehyde (MDA) assay of culture media containing liver slice tissue exposed or not to soluble egg antigen (SEA).Geoffrey, N. GobertSujeevi, K. NawaratnaMarina, HarvieGrant, A. RammDonald, P. McManus
Non-essential amino acids suppressed the effects of metformin on MDA-MB-231cells in a 2D culture and in 3D tumor spheroids. Glutamine and pyruvate weakly diminished the effects of metformin in 2D culture. Furthermore, glucose protected tumor spheroids against metformin-induced disintegration. Our ...
As HPO42−was found to alter the pH of the media, the difference in the tolerance of MDA-MB-231 and THP-1 cells to different pH levels was examined. For this, cells were incubated in media solutions at pH values equal to 5, 6, 7, 8 and 9 for 48 h then their viability determin...
MDA-MB-231 cells were placed on top of a Matrigel barrier. FVIIa and other agonists were added to a lower well that contained NIH3T3 cell–conditioned media. At the end of a 48-hour incubation period at 37°C, the number of cells that migrated across the Matrigel barrier to the ...
The aims of this study is to examine the concentration- and time-related effects of curcumin on two different breast cancer cells, MCF-7 and MDA-MB-231, and investigated the functional changes induced by curcumin treatment, as well as their relationship to the PI3K/Akt-SKP2-Cip/Kips pathway...
RNA determined by prolonged conditions of hyperglycemia (20 mM, chronic conditions) were correlated with correspondent elevated levels of TXNIP protein, we analyzed the expression of the protein in MDA-MB-231 cells grown "chronically" and stably in 20 mM glucose tissue culture media by Western ...
MB-231 cells were seeded in 6-well plates at a density of 15 × 104 cells and incubated for 24 h prior to their treatment by Av/OL for 24 h before co-culture. On the next day, cells to be labelled (donor cells) were washed with PBS and incubated in RPMI media for 1...
MDA-MB-231 and OVCAR-3 cells were loaded with 100 nM TMRM for 30 min at RT. Culture media were then replaced with PBS in order to avoid phenol red interferences, and fluorescence was immediately measured in a Victor3 microplate reader (PerkinElmer, Waltham, MA, USA). In order to ...
Fig. 1. Venn diagram of the overlap among DEGs of MCF-7 and MDA-MB-231 cells exposed to DS (2.30 µM, 72 h). The MCF-7 and MDA-MB-231 cells shared seven genes of which six genes were found in the data base. 2. Experimental design, materials and methods 2.1. Cell culture, ...