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Celecoxib is a selective cyclooxygenase (COX)-2 inhibitor that has been reported to reduce the risk of breast cancer. In our previous study, celecoxib induced apoptosis and caused cell cycle arrest at the G0/G1 phase in the breast cancer cell line MDA-MB
The use of MDA-MB-231 cells in nanoplastic research is conducted for the following reasons: this cell line is a highly aggressive, triple-negative breast cancer (TNBC) model characterized by poor differentiation and the absence of estrogen receptor (ER), progesterone receptor (PR), and HER2 (h...
(a) MDA-MB 231 cell line, (b) MCF-10A cell line, and (c) DOX cytotoxicity comparison in both cell lines. DOX: doxorubicin. Full size image The IC50 for nrCap18 on MDA-MB 231 was approximately 0.29 μg/ml, while this value for MCF-10A was 5.53 μg/ml. The IC50 of the ...
Apoptosis/cell death detection MDA-MB-231 cells transfected with a VEGF isoform or control construct were starved for 72 h in a serum-depleted medium (DMEM 1 g l−1D-glucose, 0.5% FBS). Adherent cells were rinsed with PBS and subjected to double staining with fluorescein isothiocyanat...
Full size image In MDA-MB231 cells, HGF transiently enhanced Met tyrosine phosphorylation at 30 min, similar to in MCF-7 cells (Figure 3A). However, the phosphorylation patterns of ERK1/2, Akt and c-Src were different in the two cell lines after HGF treatment (Figure 3B). The numbers...
3.1. Glycolysis and lactate release are increased in the metastatic breast carcinoma cell line MDA-MB-231 As models of breast carcinoma cells with different metastatic potentials, we used human metastatic MDA-MB-231 cells that express high levels of monocarboxylate transporter 4 (MCT4) [33], and...
(A) MCF-7 cell line and, (B) MDA-MB-231 cell line. Values are expressed as mean ± std. dev. (n = 3). Full size image LDH release assay Cell death in culture was also determined by the release of LDH into the incubation medium. The LDH release curves for MCF-7 and ...
The current study further investigates the cellular anticancer activities of an S. crispus subfraction (SCS) and its ability to modulate tamoxifen-induced effects on the breast cancer cell lines, MCF-7 and MDA-MB-231, as well as the non-cancerous breast epithelial cell line, MCF-10A....
2024, Molecular Diversity Herbal Melanin Inhibits Real-Time Cell Proliferation, Downregulates Anti-Apoptotic Proteins and Upregulates Pro-Apoptotic p53 Expression in MDA-MB-231 and HCT-116 Cancer Cell Lines 2023, Medicina (Lithuania)View full text ...